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KMID : 1140120130180010066
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Cancer Prevention Research
2013 Volume.18 No. 1 p.66 ~ p.73
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Antioxidant and Antigenotoxic Effect of Sansuyu Fruit (Corni fructus) Extracted with Various Solvents
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Lee Min-Hee
Kim Jung-Mi
Park Eun-Ju
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Abstract
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The objective of this study was to evaluate the antioxidant activities and antigenotoxic effect of Corni fructus extracted with various solvents (Ace; acetone, EtOH: ethanol, and MeOH; methanol). Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity were determined in the samples. Also antigenotoxic effect of Corni fructus was determined by measuring inhibitory effect of H2O2 induced DNA damage using comet assay in human leukocytes. The TPC of the Corni fructus extracts was in order of Ace (0.5 g GAE/100 g) ＜EtOH (2.8 g GAE/100 g) ＜MeOH (4.5 g GAE/100 g). The IC50 of DPPH RSA was significantly different between EtOH, MeOH and Ace, especially Ace (871.1 ?g/ml) is two-fold lower than EtOH (458.9 ?g/ml) and MeOH (468.2 ?g/ml), and the IC50 of SOD-like activity showed similar reduction (EtOH; 827.2 ?g/ml=MeOH; 1193.8 ?g/ml ＜Ace; 3949.2 ?g/ml). MeOH extract of Corni fructus showed the highest ORAC activity among samples. 200 ?M H2O2 induced DNA damages in human leukocytes were significantly reduced by the pretreatment with all Corni fructus extracts. Significantly high correlation coefficients were observed between TPC and antioxidant paramaters, but H2O2 induced DNA damage did not have a relationship with TPC and antioxidant paramaters. As a results, the Corni fructus extracts have antioxidant activities and antigenotoxic effect and its activities showed that there were difference between solvent extractions. Further inverstigation is needed to identify its antigenotoxic compunds.
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KEYWORD
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Corni fructus, Antioxidant, Antigenotoxic, Various Solvents
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